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OJVRTM
Online Journal of
Veterinary Research©
Volume 10
(1):61-67, 2006. Extensively redacted 2018.
In vitro Inhibition of Mastitis Pathogens by Bacteriocin RN 86 Produced by an Indigenous Lactobacillus
casei isolate
Mojgani
N, Ashtiani MP
Biotechnology department, Razi vaccine and
serum research Institute, Iran
SUMMARY
Mojgani N, Ashtiani MP., In vitro Inhibition of Mastitis
Pathogens by Bacteriocin RN 86 Produced by an
Indigenous Lactobacillus casei isolate, Onl J Vet Res., 10 (1):61-67, 2006. A broad-spectrum bacteriocin of L. casei RN 86
stable at broad pH and temperature ranges, was found to be bactericidal against
a range of mastitis-causing bacterial pathogens. Antimicrobial activity
increased 10 fold (AU/ml) after partial purification by 20% ammonium sulphate precipitation. The partially purified peptide was
bactericidal against S. aureus and S. agalactiae
and killed ~106 to 109 of actively growing sensitive cells within an hour of
incubation. The bacteriocin inhibited growth of the
sensitive strains within minutes of contact and 99.9% of the cells were killed
within an hour of incubation with 10240 AU/ml. A formulation of bacteriocin RN86 with minute concentrations of antibiotics
currently used for the treatment of bovine mastitis,
was shown to inhibit Staphylococcus aureus and Streptococcus agalactiae in vitro.
Key words: Bovine mastitis, L.casei, bactericidal, bacteriocins,
antibiotics, teat seals, in vitro.
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