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OJVRTM

Online Journal of Veterinary Research©

 (Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

 Volume 25 (10):684-694, 2021.

Effects of methamphetamine on KI-67, PPARα, PPARγ and Bax genes on

human SH-SY5Y cell proliferation, differentiation and apoptosis.

 

Marzieh Zamani-Pereshkaft1, Davood Mehrabani2,3,4*, Bahareh Borzou5, Shahrokh Zare2, Iman Jamhiri2

 

1Department of Genetics, Yazd Science and Art University, Yazd,  2Stem Cell Technology Research Center, 3Burn and Wound Healing Research Center, 4Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, 5Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran.

 

ABSTRACT

 

Zamani-Pereshkaft M, Mehrabani D, Borzou B, Zare S, Jamhiri I., Effects of Methamphetamine on KI-67, PPARα, PPARγ and Bax genes on human SH-SY5Y cell proliferation, differentiation and apoptosis, Onl J Vet Res., (10):684-694, 2021. Methamphetamine is a powerful stimulant of the central nervous system. We describe effect of the drug on cell viability, proliferation, differentiation and apoptosis in human SH-SY5Y cells. Succinate dehydrogenase activity (MTT assay) was used to determine neurotoxicity and RT-PCR for effects on expression of KI-67, PPARα, PPARγ and Bax genes in human SH-SY5Y cell proliferation, differentiation and apoptosis. Glyceraldehyde-3-phosphate dehydrogenase was used as housekeeping gene. SH-SY5Y cells appeared neuroblastic growing in clusters and clumps. Methamphetamine was associated with dose dependent reductions in SH-SY5Y cell viability Compared with SHSY-5Y controls, 1.2μM  methamphetamine increased expression of Bax gene 1.51 fold (P < 0.01). In contrast Ki-67 gene declined 0.55 and 0.41 fold (p < 0.002-0.0004). Doses of 0.6 or 1.2um metamphetamine, reduced PPARα gene 0.75 and 0.54 fold (p < 0.02-0.002) and PPARγ expression 0.06 and 0.04 fold. We find that low dose 0.6-1.2 μM methamphetamine reduced human SH-SY5Y cell viability, proliferation and differentiation and enhanced apoptosis.

Key words: Methamphetamine, Neurotoxicity, SH-SY5Y cells, Proliferation, Differentiation, Apoptosis.

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