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OJVRTM
Online Journal
of Veterinary Research©
Volume 24(12): 688-692, 2020.
Methicillin Resistant Staphylococcus aureus isolated
from surgical site patients.
Sherein I. Abd El-Moez, Sohad M Dorgham,
Eman Abd El-Aziz.
Department of Microbiology
and Immunology, National Research Center, Giza, Egypt.
ABSTRACT
El-Moez SI, Dorgham SM, El-Aziz EA., Methicillin Resistant
Staphylococcus aureus isolated from surgical site patients, Onl
J Vet Res., 24(12): 688-692, 2020. We
report methicillin resistant Staphylococcus
aureus (MRSA) from hospital patient’s pus, sputum and blood samples from
surgical sites determined by mec-A gene
quantitative-PCR. We then tested the isolates against various antibiotics and
probiotic lactobacilli from colostrum, milk and fecal samples of mares, cows or
goats. Strains were plated onto Mueller Hinton agar plates. We found mec-A gene in 6 of 7 samples. Cefobid and claforan and
probiotics B. subtilus,
L. acidophilus from colostrum of mares
and L. acidophilus from goat
colostrum were most effective against MRSA. However, L. acidophilus from Buffalo-cow milk showed no activity. We find
that mec-A gene was involved in all MRSA patients with
severe skin infections.
Keywords: MRSA;
Quantitative-PCR; mec-A gene; Antibiotic sensitivity;
Human; Probiotics.
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