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OJVRTM
Online
Journal of Veterinary Research©
Volume 25(1): 7-14, 2021.
Methicillin resistant Staphylococcus aureus (mec-A) gene isolated
from horses with toxic shock syndrome.
Sherein I Abd El-Moez 1, Ahmed FY2, Wagdy KB Khalil3, Omaima
H. Ezzo 3
1Department
of Microbiology and Immunology, 2Department of Animal Reproduction
and A.I, 2,3Department of Cell Biology, National Research Center,
Giza, Egypt.
ABSTRACT
El-Moez SA, Ahmed
FY, Khalil W, Ezzo OH., Methicillin resistant Staphylococcus
aureus (mec-A) gene isolated from horses with toxic shock
syndrome, Onl J Vet Res., 25(1): 7-14, 2021. We report
incidence of multiple drug resistant S.
aureus (MRSA) in excretions and tissues of infected and dead Arabian and
hybrid horses suffering 18.8% mortality rate due to toxic shock syndrome. A
QT-PCR for mec-A gene was performed to detect drug
resistance in S aureus and isolated 6
different strains. We tested buffalo and cow milk and goat and mare colostrum probiotics
on growth of MRSA by disk diffusion. Mec-A gene was
found in all samples of MRSA taken from horses. Cow milk probiotics L acidophilus (9.67mm), mare (8.5mm) and
goat 8.14mm colostrum exhibited most inhibition of MRSA. Buffalo milk (0mm) and
bifido-bacterium (0 -2.6mm) had no useful activity
against these bacteria. Findings suggest that probiotic L. acidophilus may be useful in controlling MRSA and dogs should not
be in contact with stabled horses.
Keywords: MRSA;
Quantitative-PCR; mec-A gene; Toxic Shock Syndrome;
Equine; Probiotic.
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