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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (2):137-147, 2022.
Herd
immunity in buffaloes by intranasal live gdhA P. multocida B:2
vaccine
Rafidah O1,2, Zamri-Saad M1,
Nasip E2, Saharee
AA1
1Faculty
of Veterinary Medicine, Universiti Putra Malaysia,
and 2Department of Veterinary Services and Animal Industry, Kota Kinabalu, Sabah, Malaysia.
Abstract
Rafidah O, Zamri-Saad M, Nasip E, Saharee AA., Herd immunity
in buffaloes by intranasal live gdhA P. multocida B:2 vaccine, Onl J Vet
Res., 26 (2):137-147, 2022. We report
herd immunity to haemorrhagic septicaemia
(HS) in large herds of buffalo given intranasal live attenuated gdhA P. multocida B:2. Three groups of 30 - 50
buffaloes from endemic location with HS outbreak reported 2 months earlier and
from non-endemic locations were grazed without vaccination. Then 30% in each
group were inoculated 5ml 106
CFU/mL live attenuated gdhA P. multocida B:2 intranasally. After
vaccination, buffaloes commingled with other animals and a booster was given 10
months after. Serum was taken from 30% buffaloes of each group prior to and every
2 month for 22 months to determine herd immune status by ELISA. The endemic or
non-endemic herd status was compared 15 non-vaccinated controls. We found (p<0.05)
higher herd antibody levels in both endemic and non-endemic areas before
vaccination. However, afte, antibodies in both areas gradually
increase and peaking (p<0.05) at 6 months then gradually falling to 10
months. Following booster dose at month 10, the herd antibody levels decreased
drastically to an insignificant (p>0.05) level as the unvaccinated group at
12-14 months before significantly (p<0.05) recovered thereafter until the
end of 22-month study period. It was concluded that field vaccination using gdhA derivative P. multocida B:2
increased the herd immunity that lasted between 8 and 10 months before a
booster dose was required.
Key words: gdhA derivative Pasteurella multocida B:2;
immune response; field buffalo.
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