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OJVRTM
© Online Journal of Veterinary Research
Volume 15(6): 492-499, 2011. Redacted 2017.
Polymorphism of toll like receptor-4 gene in Murrah
buffalo (Bubalus
bubalis).
M. Mitra, S. Taraphder and A. Verma
Department of
Animal Genetics and Breeding, Faculty of Veterinary and Animal Sciences, West
Bengal University of Animal and Fishery Sciences, Kolkata, India
ABSTRACT
Mitra M, Taraphder S, Verma A.,
Polymorphism of toll like receptor-4 gene in Murrah
buffalo (Bubalus bubalis).,
Online J Vet Res., 15(6): 492-499, 2011. Polymerase chain reaction (PCR) and
restriction fragment length polymorphism (RFLP) analysis of toll-like receptor
4 (TLR-4) gene was performed on blood samples from 102
Murrah Buffaloes. Amplified PCR fragment of TLR-4
exon 1, exon 2, exon 3.1 and exon 3.2 RFLP produced fragments of 165, 300, 478
and 409 bp, respectively. The PCR–RFLP analysis using
Dra I, Hae III
and Hinf I restriction enzymes revealed polymorphisms in
exon 1, exon 3.1 and exon 3.2 of the TLR 4 gene. Hind III did not cut any sequence. Genotypes frequencies were
0.088-0.676 for AA, 0.078-0.746 for AB and 0.049-1.000 for BB genotypes. The B
allelic frequency (0.186-1.000) was more prevalent than allele A (0.814-0.206).
Results suggest that polymorphisms in TLR-4 gene could be used as a possible
marker for identifying mastitis resistance in Murrah
buffalo.
Keywords: Bubalus bubalis, Murrah Buffalo, TLR-4 Gene, PCR-RFLP.
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