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OJVRTM

Online Journal of Veterinary Research©

 

Volume 18(10): 806-814, 2014. Redacted 2018.

Isolation and identification of Mycoplasma mycoides from goat fluids.

 

 Hossein Fattahi1, Seyed Ali Pourbakhsh2, Mahmoud Jamshidian1, Kivan Tadayon2

 

1Department of Microbiology, college of veterinary science, Science and Research Branch, Islamic Azad University, 2Mycoplasma reference laboratory. Razi Vaccine and Serum Research Institute, Karaj, Iran

 

 

ABSTRACT

 

Fattahi H, Pourbakhsh AS, Jamshidian M, Tadayon K., Isolation and identification of Mycoplasma mycoides from goat fluids, Onl J Vet Res., 18(10): 806-814, 2014. Contagious agalactia due to Mycoplasma spp causes mastitis, arthritis, kerato conjunctivitis and abortion in goats. Mycoplasma spp from 52 ear, eye and milk secretion goat samples were isolated and identified by PCR and culture. 17% samples were positive by agar culture. PCR assay of 163bp fragment of 16SrRNA gene, M agalectiae in 375bp fragment of lipoporotein gene and M. mycoides cluster in 259bp fragment of 16SrRNA gene revealed that 63% were positive for Mycoplasma-genus, 55% for M.agalectiae and 7% for M.mycoides   M.mycoides originated from nose swabs.

 

KEY WORDS: Mycoplasma Mycoides cluster, Contagious Agalactia, PCR, goats.

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