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OJVRTM

Online Journal of Veterinary Research©

Volume 19(9): 593-597, 2015.

Diagnosis of Mycoplasma Synoviae by plate agglutination and Elisa tests in commercial broiler chickens

Majid Ezatkhah¹, Maryam Amini^1*, Mehrdad Shamsaddini Bafti¹, Mojtaba Alimolaei^{1, 2}

¹Razi Vaccine and Serum Research Institute, kerman Branch,²Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

ABSTRACT

Ezatkhah M, Amini M, Shamsaddini M, Alimolaei M., Diagnosis of Mycoplasma Synoviae by plate agglutination and Elisa tests in commercial broiler chickens, Onl J Vet Res., 19(9): 593-597, 2015. Mycoplasma synoviae (MS) causes economic losses to the poultry industry through joint and/or respiratory pathology. Mycoplasmosis can be diagnosed by serum plate agglutination (SPA), hemagglutination inhibition (HI), enzyme linked immunosorbent assay (ELISA) and/or PCR. We describe serum results from SPA and ELISA tests for detection of antibodies against MS in commercial poultry flocks. 441 blood samples were collected from poultry flocks from different location in 52 to 58 day old broilers. 27% (119) were positive to SPA, but only 14% (65) to ElISA tests. ELISA has a high level of specificity whereas SPA test does not, which results in a high number of false positives using SPA. Therefore SPA results have to be reconfirmed by HI or ELISA. Results suggest that SPA should only be used for initial screening in commercial broilers and that PCR test is the most precise one.

Key words: Serologic, ELISA, SPA, Mycoplasma Synoviae.

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