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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume  29 (12): 731-739, 2025.


Effect of pH, temperature and fatty acids on growth of Mycoplasma  capricolum  

 

AZSA Zaki, H Boehnel, SH Babiker, KM Suleiman.

 

Faculty off Veterinary Medicine., University of Khartoum, Central Veterinary Research Laboratories, Khartoum, Sudan.

 

ABSTRACT

 

Zaki AK, Boehnel H, Babiker SH, Suleiman KM, E Effect of pH, temperature and fatty acids on growth of Mycoplasma  capricolum, Onl J Vet Res,  29 (12): 731-739, 2025. We report effect of pH 7 to 8 and 32 to 40°C on In Vitro growth and fatty acids in Mycoplama capricolum. We isolated palmitoleic, palmitic, linoleic, oleic and stearic fatty acids. We found that 38°C boosted palmitoleic acid, 32 to 34°C enhanced palmitic acid but 36 to 37°C reduced it and 38°C increased the levels. Higher temperatures reduced linoleic, oleic and stearic acids. Palmitoleic acid  remained stable at pH 7 to 8 whereas palmitic acid increased at pH 7 but declined at pH 8.0. Linoleic, oleic and stearic acids increased at pH 7.4 but declined at higher pH. Results suggested optimal conditions for culture were pH 7.7 and 37°C, palmetic and linoleic acids were most prominent.

 

 Key words: Mycoplasma capripneumoniae, Bioreactor, Fatty acids, Gas liquid chromatography.


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