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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and
Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (6): 329-336, 2025.
Effect of activated neutrophils
on density
and integrin expression
Spagnolo V, Giordano
A, Comazzi S, Paltrinieri S, Costa E, Sartorelli P.
Faculty of Veterinary Medicine, Milan University,
Milan, Italy.
ABSTRACT
Spagnolo V, Giordano A, Comazzi S, Paltrinieri S, Costa E, Sartorelli P. Effect of activated neutrophils on density and
integrin expression. Onl J Vet Res.,
29 (6): 329-336, 2025. Activated
bovine neutrophils (PMNs) isolated by discontinuous Percoll gradients
show changes
in density compared to resting PMNs analogous to other species. Flow cytometry
demonstrated that activated PMNs are larger and show lower density and higher
expression of integrins CD11b than non-activated
PMNs. Therefore,
under spontaneous inflammatory
conditions, results obtained by functional PMNs analysis on isolated cells may
be misinterpreted.
KEYWORDS: bovine, neutrophils,
purification, integrins (CD11b), flow
cytometry.
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