1994-2019. All Rights Reserved. Online Journal of
Veterinary Research. You may not store these pages in any form
except for your own personal use. All other usage or distribution is illegal
under international copyright treaties. Permission to
use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is exclusively
copyrighted in its entirety to OJVR publications. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors.
OJVRTM
Online Journal of Veterinary
Research©
Volume 22 (7):602-607, 2018.
Effect
of Nisin on MCF-7 tumor cell viability and apoptosis
in RPMI media.
Ghorbankhani Abbas1, Mohammadi Ali1,
Kazemipour Nasrin2, Hashempour
Sadeghian Mahdi2
1Department(s)
of Pathobiology, 2Basic Sciences, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
ABSTRACT
Abbas G, Ali M, Nasrin K, Mahdi HS, Effect of Nisin on MCF-7 tumor cell viability and apoptosis in RPMI
media, Onl J Vet Res., 22 (7):602-607, 2018. MCF-7 breast tumor cells were cultured in RPMI 1640
for 24 hours and treated with 20, 40 and 80 µg/ml nisin
for 24, 48 and 72 hours. Results were compared against MCF-7 cells in RPMI controls.
Cultured cells were analyzed by MTT, trypan blue and acridine
orange/ethidium bromide (AO/EB) assays. There was no change in percentage of apoptotic
cells in control samples. By MTT
assay we found decreased (P < 0.05) viability of cells in 20 µg/ml nisin to 72 hours whereas those in 40 µg/ml, only to 48
hours. In 80 µg/ml cell viability decreased (P <0.05) at 24 hours but did
not change by 48 or 72 hours. At 24 hours cell viability in 80 µg/ml was lowest
but in 20 µg/ml highest. However, After 72 hours
exposure at any dose of nisin we found no difference
in live cell numbers. By Trypan Blue assay,
cell viability declined greatly (P < 0.001) in 20 and 40 µg/ml nisin but most in 80µg/ml nisin at
24, 48 or 72 hours. By AO/EB assay we found time-dose increased effect of nisin (P < 0.05) on percentage of apoptotic cells. Our findings
suggest that nisin reduces viability and increases
apoptosis of MCF-7 cell line.
However type of assay appears to affect results.
Keywords: Nisin, Breast cancer, MCF-7,
antimicrobial peptide.
FULL-TEXT (SUBSCRIPTION OR
PURCHASE TITLE)