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OJVRTM
Online
Journal of Veterinary Research©
Volume
24 (1):7-14, 2020.
Summer versus Winter cardiac
glycoside oleandrin leaf content.
Rana Abdulla Salih and Ali
Aziz Alkhayyat
Department of physiology, biochemistry and pharmacology, College of
Veterinary Medicine, University of Baghdad, Iraq.
ABSTRACT
Salih RA, Alkhayyat
AA., Summer versus Winter cardiac glycoside oleandrin
leaf content, Onl J Vet Res., 24 (1):7-14, 2020. Nerium oleander leaves were collected
during July (Summer) and January (Winter). Organic
solvent soluble extract of N. oleander yielded 50 grams dried powder by soxhlet extraction, percolation at 70 °C for 72 hours to yield 10mg. We found the ratio of
extract to leaf powder weight ~4% at Winter and ~8% at
Summer being pasty dark green in Winter and crystalline light green in Summer.
At dissolution in propylene glycol the extracts appeared dark yellow in Summer and green in Winter. By HPLC we found 0.8 mg Winter
and 0.2 mg oleandrin/1g in Summer leaf samples with a
correlation coefficient of R2 = 0.98. These differences may be due
to higher levels of nonpolar components in Winter
paste samples compared with summer crystalline ones. Differences in color were
probably due to chlorophyll content. Our findings suggest that high
temperatures in summer may
reduce glycosides in this plant.
Key word: Nerium oleander, Glycoside, Leaf, Winter, Summer, HPLC.
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