©2021-2033. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.

OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 26 (8): 644-650, 2022.

Effect of gonadotropins and frozen-thawed media on In vitro ewe embryo oocytes

 

 

SM Mirshamsi1, H Karami-Shabankareh1, J Habibizad2

 

1Department of Animal Science, Razi University, Kermanshah,2Animal Science, Faculty of Agriculture, Yasouj University, Iran.

 

ABSTRACT

 

Mirshamsi SM, Karami-Shabankareh H, Habibizad J., Effect of gonadotropins and frozen-thawed media on In vitro ewe embryo oocytes,  Onl J Vet Res., Volume 26 (8): 644-650, 2022. We describe effects of gonadotropins and frozen-thawed media on ewe oocytes and zygotes. Oocytes were collected from sheep ovaries during non-breeding season for culture in media with 0.02 IU/mL pituitary, 0.075 IU/mL recombinant and/or 10 IU/mL chorionic gonadotropins or in frozen-thawed media for zygotes. Our results showed that embryo cleavage in P-FSH (61.1%) was lower (P<0.05) than rFSH-rLH (77.5%) and eCG-hCG (79.6%) We found no difference with blastocysts between pituitary (21.1%), recombinant (17.3%) and chorionic (20.5%) gonadotropins. Concerning 6 month storage and thawing our findings suggest that fresh or frozen storage media had no effect on blastocyst in P-FSH (21.1%), rFSH-rLH (17.3%) or eCG-hCG (20.5%), cleavage 73.2, 73.9, 77.4 or 78.2%,  morula 42.0 44.3, 42.8 and 48.2% or in blastocysts 17.7, 17.1, 18.2 and 18.5%, respectively. We find that non-pituitary gonadotropins and frozen-thawed culture media support subsequent embryonic development.

 

Key words: immature oocytes, maturation medium, zygotes, culture medium, brilliant cresyl blue

 

MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)