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OJVRTM

Online Journal of Veterinary Research©

Volume 20(3):210-224, 2016.

 

 Pathomorphology and PCR diagnosis of ovine pulmonary adenomatosis

 

Nikhil VM, Kumar R, Varshney KC, Nair MG*, Thanislass J1, Sivakumar M2

 

Dept(s) of Veterinary Pathology, 1Biochemistry, 2Anatomy & Histology,   Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, Pondicherry, India. *Corresponding Author:headpath@yahoo.co.in

 

ABSTRACT

 

Nikhil VM, Kumar R, Varshney KC, Nair MG, Thanislass J, Sivakumar M., Pathomorphology and PCR diagnosis of ovine pulmonary adenomatosis, Onl J Vet Res., 20(3):210-224, 2016. Ovine pulmonary adenomatosis (OPA) is a transmissible lung neoplasm of sheep induced by  Jaagsiekte sheep retrovirus (JSRV). One hundred ten lung samples from slaughtered sheep presented focal nodular or diffused consolidated OPA in single or multiple lobes. Both forms had acinar and papillary patterns of alveolar & bronchiolar epithelial proliferation lined by cuboidal to columnar epithelial cells, myxoid nodules, extensive caseation necrosis, peribronchiolar and perivascular lymphoid hyperplasia with interlobular septal thickening. Numerous alveolar macrophages but few neutrophils and mononuclear cells were present. A JSRV specific hemi-nested PCR (hn PCR) from DNA samples produced amplified product (176 bp) in OPA samples confirmed in primary and secondary PCR’s (133 bp), and samples kept in 10% formalin for 45 days. Sensitivity of U3 hn PCR in DNA samples was detected up to 107 fold dilutions in both PCR rounds.

 

KEY WORDS: Ovine Pulmonary Adenomatosis-Histopathology-Polymerase chain reaction.

 

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