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OJVRTM
Online Journal of Veterinary Research©
Volume 20(5):327-334, 2016.
Effect of insulin-like growth factor
(IGF-1) on proliferation of ovine spermatogonia stem
cells.
Mohsen Solgi, Peyman Rahimi-Feyli,
Zahra Nikousefat, Aliasghar
Moghaddam
Department of
Clinical Sciences, Faculty of Veterinary Medicine, Razi
University, Kermanshah, Iran
ABSTRACT
Solgi M, Rahimi-Feyli P, Nikousefat Z, Moghaddam A., Effect of insulin-like growth factor (IGF-1)
on proliferation of ovine spermatogonia stem cells, Onl J Vet Res., 20(5):327-334, 2016. Undifferentiated spermatogonia stem cells
(SSCs) self-renew and differentiate to produce transgenic animals and improve
reproductive efficiency in ruminants. We
describe dose-effect of insulin-like growth factor-1 (IGF-1) on In vitro proliferation of ovine spermatogonia stem cells. Spermatogonia
and sertoli cells were isolated from testes of 2-3
month old lambs by two-step enzymatic digestion and cultured for 10 days with
IGF-1 10, 40 and 100ng/ml in media or without IGF-1 as control. Cell
proliferation was evaluated at 4, 7 and 10 days. Type A
spermatogonia were identified by immuno-histochemical
staining against PGP9.5 antigen. Spermatogonia stem cells and surface area in culture with
100ng/mg IGF-1 at days 4, 7, 10 were greater (P <0.05) compared with other
treatments or controls. Treatment with 40ng/ml IGF-1 was different with other
groups (P <0.05) and 10ng/ml had no effect. Results suggest that 100 ng/ml
IGF-1 in culture medium may affect proliferation of ovine spermatogonia
stem cells.
Key words: Spermatogonial stem cells, Insulin-like growth factor -1,
ovine, PGP9.5.
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