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OJVRTM
Online Journal of Veterinary Research©
Volume 21(11):714-719,
2017.
Genetic characterization of
cytochrome oxidase I in Marghoz goat
Reza Seyed
Sharifi1, Sima Savar
Sofla2, Hamid Reza Seyedabadi*2
1Department of Animal Science, Faculty of Agricultural
and Natural Resources, University of Mohaghegh Ardabili, Ardabil, 2Animal Science Research Institute of Iran (ASRI),
Agricultural Research, Education and Extension Organization (AREEO), Karaj,
Iran, *Corresponding Author: Hamid Reza Seyedabadi,
E-mail: hseyedabady@gmail.com
ABSTRACT
Sharifi RS, Sofla SS,
Seyedabadi HR., Genetic characterization of
cytochrome oxidase I in Marghoz goat, Onl J Vet Res., 21(11):714-719, 2017. We used sequence analysis of mitochondrial DNA
Cytochrome oxidase subunit I (COXI) to identify and differentiate Marghoz goat from other breeds. Genomic DNA was isolated by
salting out and cytochrome oxidase I gene amplified by PCR. Phylogenetic trees
and pairwise calculations were determined with Mega 6 software. We found a
partial sequence of cytochrome oxidase I gene of Marghoz
goat of 1286 bp with 4 sites and 3 haplotypes.
Phylogenetic analysis of haplotype with GenBank goat
data showed Marghoz goat clustered in a separate
lineage. This is a first COX1 phylogenetic analysis of Marghoz
goat evaluated at maximum resolution with COX1 structure conserved and selected
during evolution.
Key words: mitochondrial cytochrome
oxidase I gene; genetic diversity; phylogenetic analysis; Markhoz
goat.
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