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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (7): 386-393, 2025.
Effect
of oxygen
tension on membrane transport in chondrocytes.
Milner PI, Fairfax TPA, Wilkins RJ, Gibson JS
Department of Veterinary Medicine, Madingley
Road, Cambridge and University Laboratory of Physiology, Oxford, UK.
ABSTRACT
Milner PI, Fairfax TPA, Wilkins RJ, Gibson JS,
Oxygen tension and membrane transport in chondrocytes, Onl J Vet Res 29 (7): 386-393, 2025. Articular
chondrocyte O2 tension ranges 1 to 7% but measured at 21%. We
report pHi, H+ and Na+
transport in normoxia and anoxia in chondrocytes from
articular cartilage. pHi
was measured fluorimetrically; Na+
and K+ influx were determined radioisotopically,
using 22Na+ and 86Rb+.
Resting pHi was unaltered by anoxia (6.93
+ 0.15 in oxygenated cells cf 6.89 + 0.07 in N2).
When cells were acid loaded by the ammonium prepulse
method, pH recovery and Na+/H+ exchanger (NHE) activity
were likewise unaltered by O2 tension. Amiloride-sensitive
JH was 1.47 + 0.16 mmol.(l
cells.min)-1 in air cf
1.12 + 0.25 in N2; NHE activity, measured radioisotopically,
was 65.1 + 9.6 nmol.(106 cells.h)-1 in O2 and 65.6 + 9.9 in N2.
None of these changes was significant. By contrast, Na+/K+
pump activity was markedly elevated in anoxia: ouabain-sensitive
K+ influx was 31.8 + 5.7 nmol.(106
cells.h)-1 in air and 59.6 + 9.9 in N2.
KEY WORDS: Oxygen – chondrocyte – ion homeostasis.
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