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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (3): 227-235, 2022.
Prevalence of canine enteritis parvovirus by hemagglutination and PCR tests.
S Parthiban,
HK Mukhopadhyay, K Ravikumar, PX Antony, RM
Pillai
Department of
Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal
Sciences, Puducherry-605 009.
ABSTRACT
Parthiban S, Mukhopadhyay HK,
Ravikumar K, Antony PX, Pillai RM., Prevalence of
canine enteritis parvovirus by hemagglutination and
PCR tests, Onl J Vet Res., 26 (3): 227-235, 2022. We collected swab fecal samples from 128 dogs with diarrhea/enteritis for
canine parvovirus (CPV) tests. The samples were subjected to hemagglutination (HA) and
polymerase chain reaction (PCR) assays. Of 128 samples, 47 (36.71%) had HA titre
of >=1: 32 of which 36 had titres >=1.64 considered
highly positive. HA results were confirmed on CPV rabbit hyperimmune
serum yielding 47 positive samples. The same 128 samples were then subjected to
PCR assay with CPV-2ab primer pair. We detected 68 (53.12%) yielding a single
DNA amplicon of 681 bp to confirm CPV showing that 32
negative to HA were positive by PCR. Antibodies in intestinal lumen may bind virions to prevent hemagglutination
whereas PCR can detect 103
virus PFU/gram of feces accounting for its higher
sensitivity.
KEY WORDS: Canine, parvovirus, hemagglutination, PCR.
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