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OJVR
Online Journal of Veterinary Research©
Volume 22 (1):30-37, 2018.
Detection of glycoconjugates
in porcine placental villa from different gestational periods
Sanchos G,
Merkis C, Koncurat
M.
Area de Microscopía Electrónica, Facultad
de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Argentina
ABSTRACT
Sanchos G,
Merkis C, Koncurat
M., Detection of glycoconjugates in porcine placental
villa from different gestational periods, Onl J Vet
Res., 22 (1):30-37, 2018. Porcine placenta is epitheliochorial, non invasive,
diffuse, folded and non-decidual. Since saccharides
are involved in cell-cell recognition and adhesion, their type and presence in
the placental interface is of particular interest. We describe types of glycol-conjugates
in the placental villi at different gestational periods. Nine
reproductive tracts from different gestational periods of cross bred swine were
analyzed: ±28 (n=2), ±55 (n=2), ±60 (n=1), ±70 (n=1) and ±114 days of pregnancy
(n=3). Placental structure was determined by light microscopy
and glycol-conjugates by lectin histo-chemistry with
fluorescent lectins. Sialic acid was bound during all gestational periods
whereas different oligosaccharides showed most expression in intermediate
periods of gestation. For example, α-gal, β-gal and galNAc
were present from the beginning to the end of gestation binding most at Days 55
and 70. Glucose and mannose were the most common found at every gestational
period. Our results confirm those reported in animal with epitheliochorial
placenta. The saccharides appeared heterogeneous but different in porcine
placental villa throughout gestation.
Key words: placenta, gestational periods, lectins, glycoconjugates,
porcines.
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