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OJVRTM

Online Journal of Veterinary Research© 

Volume 19(8): 486-490, 2015.


 

Polyclonal antibody from rabbit for detection of bursal disease virus

 

Amir Shabdini Pashaki1, Mehdi Aminafshar1*, Mohammadreza Safarnejad2, Seyyed Davood Hoseini3

 

1Department of Animal Science, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Plant Viruses, Iranian Research Institute of Plant Protection, Tehran, Iran. 3Razi Vaccine and Serum Research Institute, Arak, Iran.

 

ABSTRACT

 

Pashaki AS, Aminafshar M, Safarnejad M, Hoseini SD., Polyclonal antibody from rabbit for detection of bursal disease virus, Onl J Vet Res., 19(8): 486-490, 2015. Antibody for detection of Bursal disease (BD) induced in rabbits is described. Killed BD viruses were emulsified with oil for inoculation in rabbits and serum was then titrated after each of 4 inoculations. Antibody in blood was purified by affinity chromatography using a protein A column. Alkaline phosphatase was conjugated to the immunoglobulin which was then detected by double antibody sandwich enzyme-linked immunosorbent (DAS-ELISA) and dot immunosorbent assays (DIBA). These preliminary results suggest that the purified antibody could be used for detection of infectious bursal disease virus.

 

Keywords: ELISA, Infectious Bursal Disease (IBD), DIBA, Polyclonal Antibody.


 

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