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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume  29 (8): 487-498, 2025.


 Probiotics in lactic acid bacteria from chicken intestines  

 

Mojgani N, Torshizi M, Molae M.

 

Department of Biotechnology, Razi Vaccine and Serum Research Institute,c  Department of Animal Science, College of Agriculture, Tarbiat Modarres University , Iran

 

SUMMARY

 

Mojgani N, Torshizi M, Molae M., Probiotics in lactic acid bacteria from chicken intestines  Onl J Vet Res., 29 (8) : 487-498, 2025. We excised 250 chicken intestines screened for Lactic acid bacteria (LAB) by culture, morphology and biochemistry. We identified 75 isolates as LAB for probiotic of which 3 were resistant to low pH and had high bile salts. Based on biochemical reactions these strains were identified as, Lactobacillus acidophilus RNL26, L. fermentum RNL 44 and L. salivarrius RNL 49. These strains demonstrated enhanced inhibitory activity against gram positive Clostridium perfringens, Staphylococcus aureus and Listeria monocytogenes. L. fermentum RNL 44 inhibited gram negative bacteria; Salmonella enteritidis and S. typhimurium due to bacteriocin. The antibacterial effect by L. acidophilus RNL26 and L. salivarrius RNL 49 was due probably to bacteriocin as neutralized catalase treated supernatant from these strains retained antibacterial activity.  These isolates exerted In vitro cholesterol removal, aggregation, co-aggregation, and cell surface hydrophobicity.

 

Key Words: Lactic Acid Bacteria (LAB), Invitro, Antimicrobial, Poultry, Probiotic potential.


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