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OJVRTM
Online Journal of Veterinary
Research©
Volume (10):942-955,
2019.
Effect of Helicobacter pylori
antigens on hypersensitive footpad test,
interleukin-6 and gamma immunoglobin in male Albino rats.
Samir SH Al-Jabir, Ikram
AA Al-Samarraae.
Microbiology, Faculty of Veterinary Medicine,
University of Baghdad, Baghdad, Iraq.
ABSTRACT
Al-Jabir SSH, Al-Samarraae I., Effect of Helicobacter
pylori antigens on hypersensitive footpad test, interleukin-6 and gamma immunoglobin in male Albino rats, Onl
J Vet Res., (10):942-955, 2019. Groups of 6
albino male rats each were injected subcutaneous (SC) 0.5 or 1 mg/ml killed
whole cell Helicobacter pylori
sonicated antigen or 1x109 cell/ml non-sonicated antigen. A booster
shoot was given at 14 days. Controls were injected 1ml PBS. Blood was taken at
12, 28, 42 and 56 days for serum interleukin-6 and gamma immunoglobin.
After 18 days, a delayed
hypersensitivity reaction skin (DHL) test of 0.2ml sonicated antigen was done
at left footpad and 0.2ml PBS (pH 7.2) controls in the right pad and skin
thickness (mm) measured at 24, 48 and 72 hours. Compared with left pad PBS
controls, we found reduced (P < 0.05) DHL but only after 48h with 0.5mg/ml
(~-18%) or 1x109 cell/ml (~-13%). There was
no difference with higher dose 1mg/ml antigen. Compared with before test
controls pad size increased 12-16% as well as in those given only PBS. In
contrast, interleukin-6 levels increased ~21-40% in rats given the higher dose (P<0.05).
Similarly we found large increases (P < 0.05) in gamma-immunoglobulin
throughout (48-66%). Our results suggest that the lower dose sonicated antigen
may produce a greater skin reaction but the higher dose was more immuno-stimulatory.
Key Words: Helicobacter, antigens, immunity, rat. Raw data
provided.
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