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OJVRTM

 Online Journal of Veterinary Research©

Volume 22 (8):650-658, 2018.

Chemical characterization of pyocin of Pseudomonas aeruginosa and effect on promastigote Leishmania donovani In vitro.

 

Heba Saleh Shaheed, Enam Bader Faleh.

 

College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Shaheed HS, Faleh EB., Chemical characterization of pyocin of Pseudomonas aeruginosa and effect on promastigote Leishmania donovani In vitro, Onl J Vet Res., 22 (8):650-659, 2018. We identified pyocins from 25 Pseudomomas aeruginosa strains from human burn, wound and urine samples by cross streaking and RT-PCR. Bioactivity of the pyocin fractions were determined by agar well diffusion against growth of promastigote Leishmania donovani. By cross streaking test we found 18 P aeruginosa strains that produced pyocin but by RT-PCR only 12 strains wherein we identified S1, S2 and S3 genes. In well diffusion tests, pyocin inhibited growth of promastigote Leismania Donovani  (2X102-103) compared with controls without pyocin (2X106 -107). Our results suggest that 70mg/ml pyocin inhibited promastigotes of Leismania donovani.  We found S-type pyocin genes in Pseudomonas aeruginosa similar to those expressed in E coli.  

 

Key words:  Leishmania donovani, pseudomonas aeruginosa, pyocin.

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