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OJVRTM
Online Journal of
Veterinary Research©
(including
medical and laboratory Research)
Established 1994
ISSN 1328-925X
Volume 22 (10):933-940, 2018.
Production of pepsin by
bacteria (Staphylococcus sciuri) and fungi (Pythium
sp)
from soil and rumens cultured
in waste whey and brans.
Omer Dhiaa
Al-Deen Salah, Dhafer Fakhri
Al-Rawi, Harith Kamil Buniya.
College
of Education, University of Anbar, Iraq.
ABSTRACT
All-Deen Salah OD, Al-Rawi
DF, BuniyaHK., Production of pepsin by bacteria (Staphylococcus
sciuri) and fungi (Pythium sp)
from soil and rumens cultured in waste whey and brans, Onl
J Vet Res., 22 (10):933-940, 2018. Pepsin producing bacteria and fungi from 10 soil
and goat, sheep and cattle rumen content samples were identified by Biomerieux VITEK 2d and standard methods, then mixed with pasteurized whey or brans to determine
optimal pepsin production. Our results suggested that Staphylococcus sciuri and Pythium sp
produced optimal clotting at ~15 min at 50C˚, pH 2.0 in 10% whey with 1.0
ml inoculum incubated 24h yielding 4.6 units/ml pepsin.
Keyword
:
Pepsin production, Soil, Rumen, Staphylococcus
sciuri, Pythium sp,
whey, brans.
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