©2021-2033. All
Rights Reserved. Online Journal of Veterinary Research .
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR. This article may be
copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (11): 810-814, 2022.
Endogenous retrovirus subtypes in pigs.
M
Suji Prabha MSc PhD, Susan Verghese MD PhD.
Department of Microbiology, Cherian Heart Foundation, International
Centre for Cardio Thoracic and Vascular Diseases, Chennai, India.
ABSTRACT
Prabha MS, Verghese
S., Endogenous retrovirus subtypes in pigs, Onl J Vet
Res., 26 (11): 810-814, 2022. Porcine xenograft can
transmit porcine endogenous γ-retrovirus from murine leukemia and
feline leukemia viruses. We developed PCR for GAG gene region to detect
porcine retrovirus in aortic and pulmonary valves and myocardium from xenografted tissue with kidney epithelial PK-15 cell line
as positive PCR control. To distinguish retrovirus sequences, PCR for env A, env B and env C oligonuclotide primers were used. Of 50 PCR’s, retrovirus
DNA was detected in 100% envA, 82% envB, both 44%, and envC 48%. Our results suggest that retroviral DNA is present
in pig genome regardless of tissue. However, DNA retrovirus may differ from
distribution of transcriptionally active retroviruses and may not reflect
results of our analysis. Therefore, factors that influence retrovirus
expression cannot eliminate retroviruses from pig xenotransplants.
We find that common retroviruses could not determine which particles were
functional but our results suggest that retroviral DNA is consistently present
in porcine genome.
Key words: retroviruses, porcine, xenografts, zoonosis.
FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)