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OJVRTM
Online
Journal of Veterinary Research©
Volume 23 (1):56-65,
2019.
Expression of MicroRNA’s miR-10a, miR-214
and miR-29b as markers of fertility in
roosters with normal and low sperm
motility.
SH Valipour, AA Masoudi, R Vaez Torshizi.
Department of Animal
Science, Faculty of Agriculture, Tarbiat Modares University, Tehran – Iran.
ABSTRACT
Valipour SH, Masoudi AA, Vaez Torshizi R., Expression of MicroRNA’s
miR-10a, miR-214 and miR-29b as markers of fertility in
roosters with normal and low sperm motility, Onl J
Vet Res. 23 (1):56-65, 2019. In poultry, selection of
fertile or infertile males is done by assessment of sperm motility and
morphology, a method which bears weak correlation with fertility. However, microRNA’s
(miRNA’s) which regulate gene expression, play a
critical role in spermatogenesis. To our knowledge, this is a first report of expression
of miRNAs, miR-10a, miR-214, and miR-29b and sperm motility in birds by RT-PCR.
Predicted targets of the miRNAs involved in spermatogenesis were identified by in
silico search in miRDB, TargetScan
and DIANA microT uploaded into Enricher for gene
ontology analysis. Protein-protein interaction (PPI) enrichment network constructed
from STRING database revealed 77 nodes, 81 edges with a PPI p-value of 9.69e-08.
The network showed 12 major significant (P < 0.05) organic cyclic compound and
binding nodes (P < 0.05) molecular function pathways involved with
spermatogenesis. However, we found that there was no correlation between expression of the 3 miRNAs with motility of sperm (P>
0.05) in this study. We find that miRNA’ are probably not useful markers of sperm
motility.
Keywords: MicroRNA;
Fertility, Roosters.
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