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OJVRTM
Online Journal of Veterinary Research©
Volume 5:37-46,
2001. Redacted 2018.
Mitogenic reactivity of chicken intestinal intraepithelial lymphocytes to concanavalin A and its potentiation by muramyl
dipeptide and immune lymphokynes during salmonella enteriditis infection.
TR Nagaraja MVSc1, TK Raghu MVSc2, P Anand Kumar MVSc PhD3, SC Mishra MVSc
PhD4
1. Project Directorate on Animal Disease
Monitoring and Surveillance, Hebbal, Bangalore –560
024, 2. Department of Animal Husbandry and Veterinary
Services, Dudda-571 405, Mandya District, Karnataka,
3. Veterinary Biologicals Research Institute, Shanthinagar, Hyderabad – 500 048, India. 4.Division of Standardization, Indian
Veterinary Research Institute, Izatnagar, Bareilly –
243 122, Corresponding author: Dr. T.R. Nagaraja,
Research Associate, Project Directorate on Animal Disease Monitoring and
Surveillance Hebbal, Bangalore – 560 024, India,
Phone : (O) 91-080-341 9576, Fax : 91-080-341 5329, E-mail : navadhani@yahoo.com
ABSTRACT
Nagaraja TR, Raghu TK, Anand Kumar P, Mishra SC. Mitogenic reactivity of chicken intestinal intraepithelial
lymphocytes to concanavalin A and its potentiation by
muramyl dipeptide and immune lymphokynes
during salmonella enteriditis infection. Onl J Vet Res., 5: 180-199, 2001. Chicken intestinal intraepitheleial
lymphocytes (iIELs) were evaluated for their mitogenic reactivity to concanavalin
A (Con A) during early phase of Salmonella Enteritidis
(SE) infection of the host by employing MTT colorimetric blastogenesis
assay. The proliferative response of iIELs from
SE-infected chicks did not alter at 24 hr and 7 days
post infection (PI) compared to uninfected age-matched chicks. The effects of muramyl dipeptide (MDP) and SE-immune lympokines
(SE-ILK) on iIELs in uninfected and SE-infected
chicks were assessed. Administration of MDP resulted in significant increases
(P < 0.01) in mitogenic
reactivity of iIELs in uninfected and SE infected
chicks at 8 days post treatment (PT). iIELs
from uninfected and SE-infected chicks, both treated with SE-ILK (24 hr prior to infection in the latter group) showed enhanced
(P < 0.01) proliferative response at 48 hr and 8
days PT.
Key words : Intestinal intraepithelial lymphocytes ; Mitogenic reactivity; lymphokines
; Muramyl dipeptide ; Salmonella Enteritidis
; chicken