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OJVRTM
Online Journal of
Veterinary Research©
Volume 16(1): 1-7, 2012. Redacted 2017.
Detection of Brucella melitensis and pathology in seropositive goats
Plumeriastuti H1,2, Zamri-Saad M1
1Department of Veterinary Pathology and Microbiology,
Faculty of Veterinary Medicine, Universiti Putra
Malaysia, 43400 Serdang, Malaysia, 2Department of Veterinary
Pathology, Faculty of Veterinary Medicine, Airlangga University, Surabaya,
Indonesia
ABSTRACT
Plumeriastuti
H, Zamri-Saad M., Detection of Brucella melitensis and pathology in seropositive goats, Onl J Vet Res, 16(1): 1-7, 2012. We describe link between Brucella melitensis and
pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose
Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were
re-collected from the 27 sero-positive goats 2 weeks
later and subjected to RBPT for antibody titres. Uterus, mammary glands and supra-mammary
lymph nodes were examined for pathological changes, immuno-peroxidase (IP)
staining, bacterial isolation and PCR to detect B. melitensis.
Results revealed that of the 27 goats tested, 41% were Category I (antibody
titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4).
Goats in Categories 2 and 3 revealed more severe pathological changes with the
latter being most severe. All goats in Category 3 showed positive reaction to
IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%)
goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There
was strong correlation between the antibody titre, pathological lesions and
presence of B. melitensis.
Keywords: Brucella melitensis, detection, pathological changes,
seropositive goats.
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