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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (6): 317-322, 2013. Redacted 2017

Duration of replication and shedding of H9N2 virus from the trachea and feces of intranasally inoculated broilers.

 

Hadi Tavakkoli1 and Reza Kheirandish2

 

1Department(s) of Avian Medicine and 2Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

 

ABSTRACT

 

Tavakkoli H, Kheirandish R., Duration of replication and shedding of H9N2 virus in the trachea and feces of broilers, Onl J Vet Res., 17 (6): 317-322, 2013. H9N2 avian influenza subtype is endemic in chickens in Asia and is a threat to humans because of affinity with alpha-2, 6-linked sialic acid receptors in the human upper respiratory tract. The duration of H9N2 viral replication and shedding from trachea and feces of chickens after nasal inoculation is reported. 25 day-old commercial broiler chicks were inoculated intra-nasally with low pathogenic A/Chicken/Iran/SH-110/99(H9N2) virus. Titers were determined using TaqMan real time PCR after inoculation. Virus was detected in trachea 3 to 7 days and in feces up to 11 days. Maximum titers occurred on day 7 with 8876 copies in trachea and 3353919 in feces. The findings suggest that birds could pose a threat to humans for 3 to 11 days during an avian influenza outbreak.

 

Keywords: Chicken, H9N2, TaqMan real time PCR, Shedding.

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