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OJVRTM
Online Journal of
Veterinary Research©
Volume 21(3):118-125,
2017.
AA-NAT melatonin gene polymorphism in ewes
lambing out of season.
Laith
Sofian Younis, Saad Akram Hatif
College
of Veterinary Medicine, Baghdad University,
Iraq
ABSTRACT
Younis LS, Hatif
SA, AA-NAT melatonin gene polymorphism in ewes lambing out of season, Onl J Vet Res., 21(3):118-125, 2017. Three ml jugular blood samples were taken from 20
Awasi ewes lambing out season for DNA and PCR-RFLP
tests to detect polymorphisms in the AA-NAT melatonin gene. Two primer amplified
regions of the AA-NAT gene were compared with BLAST to confirm the gene and
PCR-RFLP was used to detect polymorphisms. The frequency
of G allele
(P<0.01) was greater than A Allele in ewes lambing during
October and November with no difference during September lambing. Non-seasonal reproductive frequency of the
AA-NAT-G allele was 0.625 and AA-NAT-A allele 0.375. Genotype frequencies were
25.00 for GG, 75.00 for GA and 0.00 for AA (P<0.001). Results suggest that the G allele may
be associated with non-seasonal breeding sheep whereas the A
allele with seasonal breeding sheep.
Key words:
AA-NAT gene, reproduction, ewes.
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