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Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established
1994
ISSN
1328-925X
Volume 22 (11):1068-1076, 2018.
Effect of sirolimus and doxorubicin on aberrant colorectal crypt foci
induced by azoxymethane in Balb/C
male mice.
Eman
S Hameed and Falah Muosa Kadhim AL-Rekabi
College of Veterinary Medicine, University of
Baghdad.
ABSTRACT
Hameed ES, Kadhim AL-Rekabi FM., Effect of sirolimus
and doxorubicin on aberrant colorectal crypt foci induced by azoxymethane in Balb/C male mice,
Onl J Vet Res., 22 (11):1068-1076, 2018. Authors
report effect of sirolimus and doxorubicin on aberrant
crypt foci induced with azoxymethane (AOM) in Balb/C male mice. Aberrant colorectal crypt foci stem cells
in 30 mice were induced by intraperitoneal (IP) administration of 10mg/kg Azoxymethane IP once weekly for 2 weeks. Groups of 10 mice
each were then gavaged daily 34µg/kg sirolimus (Group 1) for 4 weeks or 50mg/m² Doxorubicin
(Group 2) once weekly (after azoxymethane treatments),
for 4 weeks. Cancer controls (Group 3)
consisted of 10 mice given only AOM as described. Non-cancer controls (Group 4)
were injected 2 ml saline IP daily for 4 weeks. Mice were sacrificed for tissue
samples at day 28. Aberrant crypt foci (ACF) were counted by methylene blue and
proliferation cell nuclear antigen (PCNA) by immunochemistry and scored as low
0-4 and high levels of expression ˃ 4-12. Compared with cancer controls
(9.66 ± 1.20A), we found significant declines in ACF in mice given Sirolimus 2.66 + 0.33 or rapamycin 1.66 + 0.33, and PCNA (12 in cancer
controls and 0.8 in treated mice). However we found no difference between
treatments.
Key words: Sirolimus, Doxorubicin, Aberrant crypt foci, mice. Raw data
provided.Key words: Sirolimus,
Doxorubicin, Aberrant crypt foci, mice. Raw data provided.
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