Effect of spirulina, green tea extract and vitamin E on cadmium chloride induced testicular pathobiology

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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (1): 36-53, 2013. Extensively Redacted 2017.

Effect of spirulina, green tea extract and vitamin E on cadmium chloride induced testicular pathobiology in Sprague Dawley rats.

AsadpourR¹ DVSc, ShahbazfarAA² PhD, BeyramiM¹ DVM, Joozani JR¹ PhD.

¹Department(s) of Clinical Sciences and ²Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Iran.

ABSTRACT

Asadpour R, Shahbazfar AA, Beyrami M, Joozani JR., Effect of spirulina, green tea extract and vitamin E on cadmium chloride induced testicular pathobiology, Online J Vet Res., 17 (1): 36-53, 2013. Retention of cadmium in live tissues induces metabolic, histologic and pathologic changes. Four groups of 5 Sprague Dawley male rats each were given 200ppm cadmium chloride (CdCl₂) in drinking water with green tea extract (GTE) or 500mg/kg spirulina by gavage or 100mg/kg vitamin E intraperitoneally weekly or just water containing 200 ppm CdCl_2. The control group was provided ad libitum water. After 4 weeks, serum was collected for hormone analysis and testes tissue excised and fixed in neutral buffered formalin for microscopy. Compared with controls, CdCl₂ caused a significant decrease in sperm motility, viability and concentration, distension of interstitial blood vessels, hyperemia in testes capsule, mild edema between seminiferous tubules, necrosis of germinal epithelium and detachment from the basement membrane. Secondary spermatocytes appeared most affected by CdCl₂toxicity. Results suggested that treatments with vitamin E may have prevented histological alterations in testes. GTE appeared to provide some protection compared with spirulina treatment. CdCl₂may have induced oxidative damage in testes as GTE and vitamin E appeared to reduce lipid peroxidation and SOD and GPx levels.

Keywords: Rat, Genital system, Spirulina, Green tea, Vitamin E.

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