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OJVRTM
Online Journal of Veterinary Research©
Volume 19(7): 450-459, 2015.
Evaluation of bone marrow-derived mesenchymal stem cells for regeneration
from Guinea pigs
Aliborzi G1,2, Vahdati A1,2,
Hossini SE1,2, Mehrabani
D*3,4
12Islamic Azad University, Fars, 2Shiraz
University of Medical Sciences, Shiraz, Iran 4University of
Manitoba, Winnipeg, Manitoba, Canada.
ABSTRACT
Aliborzi G, Vahdati A, Hossini SE, Mehrabani D.,
Evaluation of bone marrow-derived mesenchymal stem cells for regeneration from
Guinea pigs, Onl J Vet Res., 19(7): 450-459, 2015.
Bone marrow from femur and tibia bones of 3 month old Guinea pigs was cultured
in Dulbecco's medium for mesenchymal stem cells (BMSC). Growth rates were
determined through 5 cell passages and compared in 24-well plates at a density
of 5×104 cells for 8 days.
Results showed that cells appeared spindle shaped through all passages,
proliferation was greatest through passage 2, and cells differentiated into
osteogenic lineage. RT-PCR showed positive
expression of CD44 and CD90 but negative expression of CD34, and karyotyping
was normal throughout.
The findings suggest mesenchymal properties of guinea pig cells for regeneration.
.
Key words: mesenchymal stem cell, bone marrow, differentiation, characterization, Guinea pig.
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