©1996-2018. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form
except for your own personal use. All other usage or distribution is illegal
under international copyright treaties. Permission
to use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is exclusively
copyrighted in its entirety toOJVR.This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors. This journal satisfies the refereeing
requirements (DEST) for the Higher Education Research Data Collection
(Australia). Linking:To link to this page or any
pages linking to this page you must link directly to this page only here rather
than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Volume 17 (1): 1-9, 2013. Redacted 2017.
Pathogenicity of subtype H9N2 virus in broiler chicks
with bursal disease
M.
Mayahi¹, M Seifiabad Shapouri², RA Jafary¹, A khodadadi, S Dibavand1, N Motamad¹*
¹Avian Diseases and ²Virology, Faculty of Veterinary
Medicine, Shahid Chamran
University of Ahvaz, Iran
ABSTRACT
Mayahi M, Shapouri MS, Jafary RA, Khodadadi A, Dibavand S, Motamad N., Pathogenicity
of subtype H9N2 virus in broiler chicks with bursal disease, Online J Vet
Res., 17 (1): 1-9, 2013. The pathogenicity
of subtype H9N2 avian influenza virus (LPAI) in broiler chicks experimentally
infected with bursal disease (BD) is described. Broiler chicks were infected with 103
CID50 of BD virus via the bursa at 1 day of age and 106
EID50 subtype H9N2 virus via intranasal and intraocular routes at day 30.
Tissue samples were collected at days 3, 7 and 11 post-infection (PI). Isolation of virus in embryonated
eggs and RT-PCR suggests that IB induced AIV in brain and liver. Moreover, virus
in trachea, conjunctiva, bursa and lung in IBD+AIV infected birds was detected
for 11 days PI. RT-PCR detected virus in the kidneys longer than the virus isolation
method. Results suggest that IBDV infection may induce greater susceptibility to
avian influenza virus infection and pathogenic effects of LPAI viruses.
Key words: Infectious bursal disease, Avian influenza, Virus shedding, RT-PCR
FULL-TEXT (SUBSCRIPTION OR PURCHASE ARTICLE)