Assessment of the shedding pattern of a chicken origin H9N2 avian influenza subtype in ostriches (Struthio camelus) using TaqMan real time PCR

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Online Journal of Veterinary Research©
16 (4): 266-273, 2012. Redacted 2017.

Assessment of the shedding pattern of a chicken origin H9N2 avian influenza subtype in ostriches (Struthio camelus) using TaqMan real time PCR

Tavakkoli Hadi¹*, Kheirandish Reza²

¹Departments of Avian Medicine and ²Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

ABSTRACT

Hadi T, Reza K., Assessment of the shedding pattern of a chicken origin H9N2 avian influenza subtype in ostriches (Struthio camelus) using TaqMan real time PCR, Online J Vet Res., 16 (4): 266-273, 2012. Avian Influenza is a contagious viral disease of globalconcern. Wide ranges of bird species are susceptible to the infection. The aim of this study was to assess the shedding pattern of chicken origin H9N2 avian influenza (AI) virus in ostriches. Five 70-day-old ostriches were inoculated intranasally with a low pathogenic A/Chicken/Iran/SH-110/99(H9N2) virus. Clinical signs and viral titer in the tracheal swab, cloacal swab and sera were determined using TaqMan real time PCR. Only two birds showed mild clinical signs. The virus was detected in the tracheal swab and cloacal swab of the infected ostriches 7 days and 11 days post-inoculation, respectively. The maximum virus titer in the tracheal swab and cloacal swab was detected on day 7 PI. In comparison with trachea, the virus was recovered in the cloaca for a longer time. Virus was not detected in serum samples. This study suggests that a weak pathogenic H9N2 AI virus of chicken origin can replicate and shed in the heterologous host without causing severe clinical disease or mortality.

Keywords: H9N2, Ostrich, Real time PCR, Shedding.

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