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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (4): 243-250, 2025.
Theileria annulata isolates (India) and cytokine gene expression.
Manuja A,
Malhotra DV, Sikka VK, Sangwan
AK, Kumar B, Nichani AK.
Department (s)
Veterinary Epidemiology and
Preventive Medicine, Biotechnology,
Veterinary Parasitology, Central Institute for Research on Buffaloes, Haryana, India. Vaccine and
Infectious Disease Organization, Saskatoon, Canada.
Manuja
A, Malhotra DV, Sikka VK, Sangwan
AK, Kumar B, Nichani AK, Theileria annulata India isolates and cytokine gene expression, Onl J Vet Res 29 (4): 243-250, 2025. We determined expression of cytokine mRNA for IL-1a, IL-1b, IL-6, IL-10, IFN-γ
and TNF-a from T. annulata Indian Hisar-1, Hisar-2, Taranagar and Anand strains by
reverse transcriptase polymerase chain reaction (RT-PCR) with cytokines primers.
Cytokines IL-1α yielded 332bp, IL-β 432, IL-6 655, IL-10 773 and
TNF-α 500bp for the isolates. However, none expressed IFN-γ. T. annulata did not produce
IFN-y as it was not expressed in any isolate. However, T. annulata can stimulate T cells to produce large
quantities of IFN-γ. Hisar-2 T. annulata yielded least expression for cytokines and
thus would have low pathogenicity. We surmise that this strain would induce
mild clinical reactions but strong immunity.
KEYWORDS: Theileria annulata, mRNA, cytokine expression, cell line
immunisation.
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