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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 30 (4): 233-242, 2026.


Prevalence of Streptococcus agalactiae in tilapia in canals, ponds, pools, rivers and reservoirs.

 

Amal AMN, Zamri-Saad M, Siti-Zahrah A, Zulkafli R, Misri S, Nur-Nazifah M, Shahidan H.

 

1Faculty of Veterinary Medicine, University Putra Malaysia, Serdang,   Malaysia2National Fish Health Research Center, Penang,   Malaysia Freshwater Fisheries Research Center, Negeri Sembilan,  Malaysia

 

ABSTRACT

 

Amal AMN, Zamri-Saad M, Siti-Zahrah A, Zulkafli R, Misri S, Nur-Nazifah M, Shahidan H. Prevalence of Streptococcus agalactiae in tilapia  in canals, ponds, pools, rivers and reservoirs.  Onl J Vet Res, 30 (4): 233-242, 2026. We isolated Streptococcus agalactiae from tilapias kept in fast flowing irrigation canals, slow flowing ponds and pools, rivers and flowing reservoirs. Over 24 months 713 tilapias were collected from canals, 708 in ponds, 633 in pools, 2,610 in rivers and 1,729 from reservoirs. Tilapias were kept in floating net cage-culture system and dissected for brain, eye and kidney streaked directly onto blood agar for Gram stain, API 20 STREP kit, Slidex Strepto-kit and PCR. We isolated Streptococcus agalactiae in all water samples but found differences (p<0.05) on prevalence in fish in reservoirs (12.50%) with water flow 0.006±0.003 cm/seconds compared with rivers with flow of 0.25±0.24 cm/seconds, pond (0.70%), canal (0.28%) and pools (0.17%). We recorded (p<0.05) higher mortality in tilapias kept in reservoirs in temperatures >29.510C 10-30 cm and 100-350g. We found correlations (r=0.9312, P=0.0008) between S. agalactiae and mortality n reservoirs with Staphylococcus spp. Findings suggest that water temperature, rate of water flow and size of fish may affect prevalence of S. agalactiae in tilapia.

 

Key words: Streptococcus agalactiae, Tilapia, canals, pools, ponds, rivers, reservoirs.


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