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OJVR
Online Journal of Veterinary Research©
Volume 22 (1):7-14, 2018.
Phylogenetic
analysis of Toll Like Receptor 4 gene in cattle.
1Zahra
Biranvad, 2*Ziaeddin Mirhoseini, 3*Mostafa Ghaderi-Zefrehei, 4Arash
Fazeil, 2Seyed Hossein Hosseini Moghadam, 5Kianoush Zarrin
Kavyani
1PhD student, Guilan University, Rasht, 2Professor in
molecular genetics, Guilan University, Rasht, 3Assistance
Professor in systems biology, Yasouj University, Yasouj, 4Assistance Professor in molecular
genetics, Ilam University, Ilam,
5Faculty member Ilam
University, Ilam, Iran. *Corresponding author: Ziaaldin Mir Hosseini, Email: szmirhosseini2015@gmail.com
ABSTRACT
Biranvad Z,
Hosseini ZM, Ghaderi-Zefrehei M, Fazeil
A, Hosseini Moghadam SH, Zarrin
Kavyani K.,
Phylogenetic analysis of Toll Like Receptor 4 genes in cattle. Onl J Vet Res., 22 (1):7-14, 2018. Detection of candidate
genes involved in immune response in cattle may assist in recognizing species
specific response to pathogens. Toll-like receptors (TLRs) have a vital role in
disease resistance through their recognition of pathogen associated molecular
patterns. We describe nucleotide variation and phylogenetic analysis of TLR4
gene in 29 species extracted from NCBI. Sequences were aligned by Clastalw and a similarity, distance and phylogentic
tree was constructed with Megalign. cDNA sequence alignment for TLR4 revealed sequences of high
similarity and one small region, possibly a mutation. Results suggested high
variation of nucleotides among sequences. Ceratothorium
simum simum showed 99%
similarity and Felis silvestris
catus 97.3% similarity, but Mus musculus only 21.4% with Homo sapiens. Cattle
with allele A in T4CRBR1 showed lower somatic cell
score than allele B (P < 0.05). Higher variability was identified in the
extracellular region of the TLR4 protein, accounting for 88.79% (AA). Findings
showed that TLR4 in Equus caballus was
very close to Bos Taurus and could possibly be used for
selecting mastitis breeding programs for cows.
KEYWORDS:
TLR4,
Phylogenetic analysis, cattle.
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