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OJVRTM
Online Journal of Veterinary Research©
Volume
22 (6):486-494, 2018
Prevalence and identity of Toxocara canis isolates in stray dogs (Iraq).
Noor Idan
Jarad, Athmar Kdair Abbas.
Department of Parasitology /College of Veterinary Medicine/University
of Baghdad/Iraq.
Jarad NI, Abbas AK., Prevalence and identity of Toxocara
canis isolates in stray dogs (Iraq). Onl J Vet Res., 22 (6):486-494, 2018. We determined prevalence of Toxocara
canis in Southern Iraq during January to September
2017 in 270 stray dogs by molecular and microscopic methods. Effect of gender,
age and season on prevalence was recorded. By fecal flotation method we found
~23% to be infected of which 23.7% were
males and 22.8% female with no statistical difference. Prevalence peaked at 40% during February with temperature of ~26º C and humidity of ~30%. Incidence was higher (P < 0.05) in younger dogs (34.9%) compared with adults (15.8%). By PCR, prevalence
was 58% with 10 T. canis identical isolates (accession no. KF577855.1) as found in Iran, while identity of 99% for T.canis3
and 4. Results revealed that the Internal Transcribed Spacer (ITS1) regions sequence of the 10
isolates showed 99% homology with reference sequences of T. canis from China
(accession no. JF837169.1).
Keywords: Prevalence, Toxocara
canis ,
Isolates, risk factors, PCR, Phylogeny.
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