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OJVRTM
Online Journal of Veterinary Research©
Volume 16 (4): 274-283, 2012. Extensively Redacted 2017
Prevalence
of Toxoplasma gondii
in sheep and goats detected by ELISA by direct and nested-PCR.
F.
Moazeni Jula1, G. Moazeni
jula1, N. Nowzari2, A. Kavari1, H. Hashemzadeh Farhang3
1Razi Vaccine and Serum Research Institute, Shiraz branch, 2Departments
of Parasitology, Veterinary Organization, Shiraz, and 3Parasitology,
School of Veterinary Medicine, Azad University of Tabriz, Tabriz, Iran.
ABSTRACT
Jula FM, Jula
GM, Nowzari N, Kavari A, Farhang HH., Prevalence of Toxoplasma gondii
in sheep and goats detected by ELISA with direct and nested-PCR, Onl J Vet Res., 16 (4): 274-283, 2012. Infection rates of toxoplasmosis in sheep and
goats detected with ELISA in conjunction with direct and nested PCR methods are
reported. T. gondii DNA in blood, cotyledon
and brain of fetuses was detected using 4 pairs of primers, 18SrRNA, ITS-1,
(Tg1,Tg2) and (Tg3,Tg4). Antibodies against T. gondii were found in 18.3% serum samples. T. Goondii DNA was detected in 69% fetal brain, 23%
cotyledon and 14.7% blood samples. Prevalence was higher in sheep (24.8%) than
goats (10.6%), in adult sheep and goats compared with young animals, and in
females (19.2%), compared with males (10.5%). The presence of T. gondii in blood, fetal tissues and neonatal specimens
suggests active or congenital infection.
Key words: Toxoplasma gondii,
ELISA, PCR, sheep, goat.
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