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OJVRTM
Online
Journal of Veterinary Research©
Volume 17 (3): 143-150, 2013. Extensively redacted 2017
A serological, molecular and histopathological survey of Toxoplasma gondii
infection in free ranging chickens
Mojaver
S1, Namavari M2, Sayari M1, Hosseini MH2, Mansourian M2, Kargar M1,
Sadegh-zadeh S 2, Hayati
M2
1 Department of
Pathobiology, School of Veterinary Medicine, Shiraz University, and 2
Razi Vaccine and Serum Research Institute, Shiraz,
Iran
ABSTRACT
Mojaver S, Namavari M, Sayari M, Hosseini
MH, Mansourian M,
Kargar M, Sadegh-zadeh,
S, Hayati M., A serological, molecular and
histopathological survey of Toxoplasma gondii
infection in free ranging chickens., Onl J Vet
Res., 17 (3): 143-150, 2013. Prevalence of Toxoplasma
gondii in free-ranging chickens (Gallus domesticus) is related to environmental contamination by T.
gondii oocysts. Blood, brain tissue and sera were
taken from 150 birds to determine the prevalence of T. gondii
infection. Sera was tested by a modified agglutination
test (MAT). Brain samples of seropositive cases were further analyzed by
molecular and immunohistochemistry (IHC)
tests. Antibodies to T. gondii were found in 6% of the serum samples and 3%
brain by PCR and IHC tests. Results suggest widespread exposure of free range
chickens to T. gondii in Southern Iran.
Keywords: Toxoplasma gondii; Chickens; Seroprevalence;
Molecular; Immunohistochemistry.
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