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OJVRTM
Online
Journal of Veterinary Research©
Volume
23 (4):285-298, 2019.
Toxoplasma gondii
and OP5 and GRA6 virulence factors isolated from ovine blood and muscle exudate.
Sahad MK Al-Dabbagh, Haider MA Al-Rubaie.
Department of Parasitology, College of
Veterinary Medicine, University of Baghdad -Iraq
ABSTRACT
Al-Dabbagh
SMK, Al-Rubaie HMA., Toxoplasma gondii and OP5 and GRA6 virulence
factors isolated from ovine blood and muscle exudate, Onl
J Vet Res., 23
(4):285-298, 2019. We determined incidence of T Gondii in 48 blood and raw meat sheep samples by
RT-PCR. B1 gene in T gondii
ROP5 and GRA6 virulence factors by location, age, gender and season are also
reported. We found T gondii
in ~19% blood samples with no difference between ROP5 and GRA6 expression. Age,
gender, location or season had no effect on incidence for T Gondii but found we found significant
(P < 0.05) differences with ROP5 (over-expression) and GRA6 gene (down
regulation). In meat, we found 33% samples infected by T Gondii, but no difference in ROP5 and
GRA6.
Key word: Toxoplasma gondii;
gene expression; virulence factors ROP5 and GRA6; sheep.
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