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OJVRTM
Online Journal of Veterinary Research©
Volume
11 (3): 5-13,
2007. Redacted extensively 2018.
Trichinella
spiralis larval antigen diagnosis in horses
Hassan SE, Abdel-Rahman EH, Ghazy AA
Department of Parasitology
and Animal Diseases, Veterinary Research Division, National Research Center, Post Box
12622, 33 El-Tahrir
Street, Dokki,
Giza, Cairo, Egypt
ABSTRACT
Hassan SE, Abdel-Rahman EH, Ghazy
AA, Trichinella spiralis larval antigen diagnosis in
horses Onl J Vet Res., 11 (3): 5-13, 2007. An affinity
purification process using CNBr-Sepharose 4B column
chromatography was done to purify Trichinella
spiralis crude larval extract.
The process resulted in a bound fraction with 6 bands of 25.7, 36.8, 58.8,
77.9, 150.8 and 174.0 KDa compared with 12 bands with
crude larval extract (16.6- 174.0 KDa) determined by
SDS-PAGE). Only four bands (25.7, 36.8, 58.8 and 174.0 KDa)
of the isolated fraction were immune-reactive with T. spiralis positive horse sera by Western blot
analysis. The bound fraction detected trichinellosis
in horses of different ages, sexes and breeds with 86.9% by ELISA, 64% with
crude larval extract
and 52% by unbound fraction (52.0%). ELISA could be used for herd
surveillance programs and epidemiological studies of trichinellosis
in horses.
Keywords: Trichinella spiralis; Horse; Affinity purification; ELISA; Western Blot.
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