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OJVR
Online Journal of Veterinary Research©
Volume 14 (1):8-17, 2010. Redacted
2017.
RNA Interference of Trypanosoma congolense P74 encoding putative ABC1 family protein.
Waren N. Baticados1*(DVM, PhD), Noboru
Inoue2 (DVM,PhD),
Chihiro Sugimoto3 (DVM, PhD), Hideyuki Nagasawa2
(DVM, PhD) and Abigail M. Baticados1 (DVM)
1College of Veterinary Medicine,University
of the Philippines Los Baños, Laguna; 2National
Research Center for Protozoan Diseases, Obihiro
University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido
8555, Japan and 3 Laboratory of Infectious Diseases, Graduate School
of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
ABSTRACT
Baticados WN,
Inoue N, Sugimoto C), Nagasawa H, Baticados AM, RNA Interference of Trypanosoma congolense P74 encoding Putative ABC1 Family Protein. Online J Vet Res 14 (1):8-16, 2010. Trypanosoma congolense RNA interference cell lines were obtained and functional
analysis of Trypanosoma
congolense P74
gene was performed using double-stranded RNA interference. The present study strongly indicates
and further verified the data of the previous research that, P74 gene is stage-specific and the
expression is developmentally up-regulated in Trypanosoma congolense bloodstream forms.
The inability to determine the function due to the stage-specific
expression of the gene still contributed to the understanding of the nature of
the gene and double- stranded RNA interference
in trypanosomes. The study further stresses
that T. congolense
is still amenable to double-stranded RNA
interference and is an excellent model species to conduct laboratory studies in
stage-specific gene expression.
Key words: Double-stranded RNA
Interference, Trypanosoma congolense, putative
ABC1 family protein, procyclic form
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