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OJVRTM
Online Journal of Veterinary Research©
Volume 17(7):370-382, 2013, Redacted 2017.
Molecular and
pathological characterization of a highly virulent infectious bursal disease
virus (vvIBDV).
Vahid Reza Ranjbar1,
Ali Mohammadi2*, Habibollah Dadras1
1Departments
of Avian Medicine and 2Pathobiology, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
ABSTRACT
Ranjbar VR,
Mohammad A, Dadras H Molecular and
pathological characterization of a highly virulent Infectious bursal disease
virus (vvIBDV), Onl J Vet
Res., 17(7):370-382, 2013.
Infectious bursal disease virus (IBDV) causes an acute highly contagious
disease in young chickens. Strains of recently isolated IBD viruses from sick
and dead birds of IBD field outbreaks were characterized. The partial region of VP2 gene from IBDV field isolates were sequenced
to assess genetic characteristics, and select viruses for further experimental study.
The nucleotide sequence of the RT-PCR fragment was determined and submitted to GenBank (ShirazIBD isolate;
Acc.no. JX983160). Then, 10 day old embryonated SPF
eggs were inoculated with a field isolate virus. At 7 days post-inoculation
(PI), embryos were examined for gross IBD lesions and RT- PCR test. Haemorrhages were seen on the CAM, skin and toe joint of
the embryos. Spleens were enlarged, pale and had small necrotic foci. These
lesions were severe in the early passages of the virus. Twenty one days old
commercial chickens were inoculated with positive IBDV field bursal sample (groupA), CAM and embryo homogenate (groupB)
and distillated water for control group (groupC). The
birds were sacrificed three days after inoculation and bursa were collected for
RT-PCR and histopathological examinations. Produced bands by RT- PCR methods in
the infected tissue samples including homogenized bursal samples, CAM and
chicken embryos were about 743bp. Histological evaluation of the bursa showed
marked bursal lymphoid necrosis and depletion in both of the infected groups.
According to these results, the isolated virus was diagnosed as vvIBDV.
Key words. IBDV, VP2 gene, GenBank, nucleotide sequence, RT- PCR
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