©1996-2019. All
Rights Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright
treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety
to OJVR. This article may be copied once but may not be, reproduced or
re-transmitted without the express permission of the editors. This journal
satisfies the refereeing requirements (DEST) for the Higher Education Research
Data Collection (Australia). Linking: To link to this page or any pages linking
to this page you must link directly to this page only here rather than put up
your own page
OJVRTM
Online Journal of Veterinary Research©
Volume 20(11):712-719, 2016.
Effect of probiotic Bacillus subtilis IS02
of Litopenaeus vannamei shrimp on pathogenic Vibrio harveyi.
Mahjoub M1*, Mirbakhsh M1, Afsharnasab M2, Kakoolaki S2, Hosseinzadeh,
S3.
1Iranian Fisheries Sciences Research Institute, Agricultural
Research, Education and Extension Organization (AREEO), Tehran, Iran, 2Department
of Aquatic Animal Health and Diseases, Iranian Fisheries Research Organization 3Department
of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz,
Iran.
ABSTRACT
Mahjoub M, Mirbakhsh M, Afsharnasab M, Kakoolaki S, Hosseinzadeh, S. Effect of probiotic Bacillus subtilis IS02
of Litopenaeus vannamei shrimp
on pathogenic Vibrio harveyi, Onl J Vet Res., 20(11):712-719, 2016. Effect
of B. subtilis IS02 on growth of Vibrio harveyi was tested in rearing salt water at 0
(controls), 106, 107 and
108 CFU ml-1 every 3 days from stage 1 larvae
to end. Shrimp were challenged for 10 days with V. harveyi at 105 CFU
ml-1 for 5 days and 107 CFU ml-1 from
day 6 to 10. Mortality in shrimp given 108 CFU ml-1 B.
subtilisIS02 was 6.9% and 40.6% in controls. Bacterial counts on TSA,
total vibrio, green and yellow colonies on TCBS were lower
(P<0.05) in the 108 CFU ml-1 treatment
group. Bacilluscounts on MYP agar in the
treatment groups were higher (P<0.05) compared with controls. Bacterial
counts were lower in treated samples. Vibrio were not
found in muscle tissue of shrimp treated with probiotics. Co-cultivation
of V. harveyi with B.
subtilis IS02 and cell free extracts inhibited V. harveyi (P<0.05) compared with controls.
Highest inhibition occurred in 108 CFU ml-1 B.
subtilis IS02 cell-free extract and lowest in 106.of same.
Results suggest that 108 CFU ml-1of probiotic, B.
subtilis IS02 inhibit pathogenic V. harveyi in vitro and in vivo.
Key
words: Bacillus subtilis,
probiotic, Litopenaeus vannamei, Vibrio harveyi
FULL-TEXT(SUBSCRIPTION OR PURCHASE TITLE $25USD)