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OJVRTM

Online Journal of Veterinary Research©

Volume 23 (11):1075-1083, 2019.

A survey of Velogenic and lentogenic Newcastle paramyxoviruses in Iraq.

Hassan AR Flaicha, Aida BA AL-Dulaimy.

Microbiology, Faculty of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.

ABSTRACT

Fleja HAR, AL-Dulaimy ABA., A survey of velogenic and lentogenic Newcastle paramyxoviruses in Iraq, Onl J Vet Res., 23 (11):1075-1083, 2019. Sixty lung, trachea, liver, spleen and brain of chickens with Newcastle Disease Virus (NDV) were inoculated into allantoic sac of 10 day embryonated chicken eggs dying after 24h. Embryos were harvested for allantoic fluid passaged twice in chicken embryos to increase virus concentration. RT-PCR confirmed paramyxovirus serotype-1. The allantoic NDV was propagated in chicken embryo fibroblast (CEF) cell culture to determine cytopathic effects at 24h. The CPEs was characterized by syncitia formation, vacuolation and degeneration of cells. In-vivo assessment of the virulence of NDV was done by Intracerebral Pathogenicity Index (ICPI) test. Each of six samples was inoculated with ten 24-40h old chicks. We found 4 velogenic viruses (1.58, 1.575, 1.525 and 1.61) and two lentogenic ones (1.025 and 0.75).

Keywords: Newcastle, virus, virulence, pathogenicity, chicken, embryo, Iraq.