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OJVRTM
Online Journal
of Veterinary Research©
Volume
25(1): 39-46, 2021
Effect of Withania
somnifera on
HepG2 cell mercury induced toxicity.
Vivek Singh, Anu
Rahal, KP Singh and AH Ahmad
Department of Pharmacology
and Toxicology,College of Veterinary & Animal Sciences,G.B.Pant University of Agriculture &
Technology, Pantnagar
India
ABSTRACT
Singh V, Rahal A, Singh KP, Ahmad
AH., Effect of Withania somnifera on
HepG2 cell mercury induced toxicity, Onl J Vet Res., 25(1): 39-46, 2021. We report in vitro effect of withania
somnifera extracts on mercury toxicity in Hep G2 cell line. MTT cell viability
assay showed that mercury produced dose dependent cytotoxic IC50 of
36.41±1.18 μM after 12 h of exposure. We found increased (P < 0.05) viability in
cells pre-incubated with extract or incubated simultaneously with mercury. Compared
with 25μM mercury controls, 64.66±1.39% viability increased to 66.01± 2.95%
in 2.5, 67.40±3.66 in 5, 70.85±3.82 in 7.5 and 72.30±2.96 % in 10uM mercury but
65.91±3.04, 68.01±3.14, 73.44±4.61 and 74.37±3.77 % when cells were treated
with Withania
extract 6h before challenge with mercury chloride. Similarly in cells treated
with 50μM HgCl2 viability was 36.80±1.87 % which increased to
38.41±1.84, 39.37±1.03, 40.60±1.91 and 44.25±1.91 % in
2.5, 5, 7.5 and 10 μg/ml Withania extract given
simultaneously or 39.08±2.41, 40.23±1.15, 42.33±1.90 and 46.46±2.42% when cells
were treated with extract 6h before challenge of mercury chloride. We find that
W. somnifera root extract administered 6h
prior or simultaneously with mercury chloride challenge may increase Hep G2 cell viability.
Keywords: Withania: Hep G2; mercury; cell
viability.
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