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OJVRTM
Online
Journal of Veterinary Research ©
Volume 14 (2): 208-11, 2010. Redacted 2017.
Genomic
isolated from pigs and humans in Greece.
Anastassios Ioannidis BSc, MD, PhD1, Chryssoula
Nicolaou MD, PhD 2, Nektaria
Kechagia MD, PhD 3 and Stylianos Chatzipanagiotou MD, PhD 2,*
1University of
Peloponnese, Faculty of Human Movement and Quality of Life Sciences, Department
of Nursing, Sparta., 2Athens Medical School, Aeginition
Hospital, Department of Biopathology and Clinical
Microbiology, and 3Athens Medical School, Department of Medical
Microbiology, Athens, Greece
ABSTRACT
Ioannidis A, Nicolaou C,
Kechagia N, Chatzipanagiotou
S, Genomic DNA Pulsed Field Gel Electrophoresis profiles of Yersinia enterocolitica Serotype O:3/Biotype
4 Isolated from Pigs and Humans in Greece. Onl J Vet
Res., 14 (2): 208-11, 2010. Pig strains (116) of Yersinia enterocolitica
serotype O:3/biotype 4 were isolated from slaughterhouses in Greece and tested
for XbaI restriction fragment length
polymorphism (RFLP) profiles by pulsed field gel electrophoresis (PFGE). The
genetic profiles from pigs were compared to those from human clinical isolates
of the same bio-serogroup. PFGE analysis revealed 2 different genetic profiles
strain A1 (99.14%) and strain A2 (0.86%). A1 was similar to the unique genetic
profile described in human clinical isolates previously. As this profile was
derived exclusively from the pigs, this result supports the assumption that
pigs seem to be the main reservoir of human pathogenic yersiniae.
Keywords:
Yersinia enterocolitica; pigs; human; RFLP; PFGE.
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